Table 1.
RNase A dimer | RNase A dimer | Dimer + Congo Red | |
---|---|---|---|
Cryogenic protectant | — | 25% glycerol | 25% glycerol |
Temperature | Room temperature | Cryo | Cryo |
Wavelength, Å | 1.54 | 1.54 | 1.54 |
Resolution range, Å | 40–2.1 | 40–2.8 | 40–2.5 |
Total reflections | 30,428 | 14,830 | 20,706 |
Unique reflections | 16,601 | 6,766 | 9,588 |
Completeness, % | 96.0 | 95.9 | 97.7 |
Rsym*, % | 9.7 | 10.1 | 8.7 |
Space group | P32 | P32 | P32 |
Unit cell constants | a = b = 57.0 Å | a = b = 56.3 Å | a = b = 56.2 Å |
c = 81.4 Å | c = 80.7 Å | c = 80.3 Å |
Rsym = Σhkl Σi |I(hkl)i − 〈I(hkl)〉|/Σhkl Σi 〈I(hkl)i〉.