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. 2001 Jan 1;107(1):99–109. doi: 10.1172/JCI10501

Figure 11.

Figure 11

Flagellin is a potent inducer of IL-8 secretion, does not drop transepithelial electrical resistance (TER), and is essential for IL-8 secretion induced by S. typhimurium. (a) Model epithelia were basolaterally exposed to indicated concentration of LPS, flagellin, or TNF-α. IL-8 secretion was assayed 5 hours later. (b) Model epithelia were exposed (basolaterally) to HBSS (Neg), PIF (1:40 of sample used in Figure 4), TNF-α (20 ng/ml), or C. difficile toxin A (Toxin A). TER was measured as described in Methods. (c) Model epithelia were apically colonized for 45 minutes with live indicated wild-type (WT) S. typhimurium or indicated mutant strain. Nonadherent bacteria were removed by washing three times with HBSS. IL-8 secretion was measured 5 hours after initial exposure to bacteria. Flagellin-deficient strain (fliC–/fljB–) transformed with plasmids encoding fliC and fljB is indicated by fliC–/fljB–/fliC+ and fliC–/fljB–/fljB+, respectively.