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Flagellin-deficient S. typhimurium do not release an IL-8 inducing bioactivity. Bacterial supernatants were isolated (as described in Figure 4) from wild-type (WT) S. typhimurium or mutant strains lacking fliC and/or fljB (“–” indicates the gene that is missing). Supernatants were then diluted as indicated, applied basolaterally to model epithelia, and IL-8 secretion assayed 5 hours later.
