Figure 9.

Flagellin rapidly appears in the basolateral supernatants of apically infected model epithelia. Model intestinal epithelia were apically exposed to indicated bacteria. Basolateral epithelial supernatants were isolated and Western blotted for flagellin. (a) Supernatants were isolated at the indicated time after addition of S. typhimurium. Isolated surface flagellin from S. typhimurium expressing FliC or FljB and PIF were directly Western blotted (i.e., not exposed to epithelia) to serve as positive controls. (b) Supernatants were isolated 1 hour after exposure to indicated organism or purified flagellin (100 ng/ml).