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. 2001 Jan 1;107(1):111–120. doi: 10.1172/JCI10741

Figure 4.

Figure 4

T3 blocks α-MSH–mediated upregulation of the hTRH promoter. (a) 293T cells were cotransfected with expression vectors for the TRβ isoforms. The cells were incubated with either 100 nM α-MSH alone or with α-MSH and 100 nM T3. The data are expressed as fold stimulation from untreated cells. (b) EMSA was performed with radiolabeled Site 4 and CREB-transfected nuclear extract in the presence or absence of in vitro translated hTRβ2. (c) GST-TRβ1 or GST-TRβ2 was incubated with radiolabeled SRC-1 or CREB either in the presence or absence of 1 μM T3. Lane 9, CREB (C) input. Lane 10, SRC-1 (S) input.