Figure 1.
Protocols for treatment of normal mice and STZ-induced diabetic mice with adenovirus. Protocol 1 was used for normal mice treated with adenovirus. Protocol 1a: Time course of overexpression of double mutant 6PF-2-K/F-2,6-P2ase in normal mice. Immunosuppressant injection (IM) started at day –2. Samples were collected (SP) on day 0 (no adenovirus infusion) and days 3, 5, and 7 (after adenovirus infusion; n = 2). Protocol 1b: Normal mice were divided into three groups (n = 6) and treated with different adenovirus. IM was started on day –2; adenovirus (AD) was infused on day 0. Samples were collected (SP) on day 7. Protocol 2 was used for diabetogenesis and adenovirus infusion. Saline control mice (n = 6) only received citrate buffer (SAL) on day –7, and samples were collected on day 7. Mice were made diabetic by STZ injection on day –7. IM started on day –2, and AD was given on day 0. Samples were collected (SP) on day 7 (n = 6).