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Specificity of series IPF isolation. (A) A mixture of synthetic IP standards, IPF-I, IPF2α-III, and 12-iso-PGF2α (a series IV IPF) analyzed by GC/MS. (B) The same mix was added to 100 μl of urine and subjected to lactonization, SPE, TLC, saponification, and derivatization are as described in the text.
