Figure 2.

Effects of linker length and mutated T7 RNAP in the termination pattern with TMR-3′-dUTP. Sequencing reactions were performed with human thyroid-stimulating hormone β-subunit unpurified PCR products for the template. Wild-type or mutated F644Y T7 RNAP for enzyme, C₁ or C₄ linker arm for dye-3′dNTP were tested, respectively. PPase was added to all reactions. All peaks are shown on the same scale. (a) Combination of C₁ linker and wt T7 RNAP. (b) Combination of C₁ linker and mutant T7 RNAP. (c) Combination of C₄ linker and wt T7 RNAP. (d) Combination of C₄ linker and mutant T7 RNAP. Arrows indicate false peaks.