Figure 3.

(A) Improvement of peak uniformity by use of PPase. Sequencing reactions were performed in the absence or presence of PPase. Minus or plus indicates the absence or presence of PPase. All peaks indicating termination patterns with TMR-3′-dUTP are shown on the same scale. The arrows indicate sites relatively sensitive to pyrophosphorolysis a. Peak degradations in sensitive sites were prevented by PPase b. (B) Elimination of peak compression by use of 7-deaza-GTP in place of GTP. The arrows indicate two compression sites in human TSH cDNA using GTP as the substrate a. Compressions in the corresponding sequence were eliminated by use of 7-deaza-GTP for the substrate b.