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. 1998 Mar 31;95(7):3461–3466. doi: 10.1073/pnas.95.7.3461

Figure 4.

Figure 4

In vivo competition assay for mMZF-2-mediated transactivation. (A) Schematic representation of the mMZF-2 derivatives used as competitors. (B) Inhibition of mMZF-2-mediated transactivation by competitors. LGM-1 cells were cotransfected with 4 μg of the reporter plasmid pBPA-4xF113B-Luc, 3 μg of the effector plasmids pEF-BOS (BOS, no insert), mMZF-2, ΔLeR, ΔN117 or KM3, and 3 μg of the competitor plasmids of pEF-BOS, N1–124, N1–238, N1–415, and N235–415. As a control, 1 μg of β-galactosidase plasmid (pSV-β-gal) was also included. At 24 h after transfection, the luciferase activities were determined as described above, and normalized with the value of β-galactosidase activity. The luciferase activity is expressed relative to that obtained with the empty effector plasmid (pEF-BOS). The average values from at least two independent experiments are shown with standard deviations (thin bars).