Figure 5.

EMSA of preadipocyte and adipocyte nuclear extracts with the oligonucleotide sequence in the human C/EBPα gene that corresponds to the CUP-1 binding site in the mouse gene. EMSA was performed with nuclear extracts from 3T3-L1 preadipocytes (P; day 0) or adipocytes (A; day 7) with an oligonucleotide sequence in the human C/EBPα gene that corresponds to the CUP-1 binding site in the mouse C/EBPα gene. Where indicated, nuclear extract was treated with antibody against hAP-2α (AP-2) or preimmune serum (PI) before incubation with the labeled oligonucleotide probe. Two arrows on left indicate complexes formed with rAP-2; arrow on right the complex formed with preadipocyte nuclear extract.