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Transinhibition by AP-2α1 of reporter gene expression mediated by the promoter of the C/EBPα gene in 3T3-L1 adipocytes. Day 3 3T3-L1 adipocytes were transiently cotransfected with an AP-2α1 or control (lacking an AP-2α1 cDNA insert) expression vector and either a promoterless (lacking the C/EBPα promoter) or C/EBPα promoter-luciferase construct. After 48 h, luciferase assays were conducted on cell extracts. Results are from five independent experiments.
