Table 1.

Purification of CUP from 3T3-L1 preadipocytes

Step	Protein, μg	Activity, arbitrary units	Specific activity, units/μg	Yield, %	Purification, -fold
Cells*	80,000	—	—	—	—
Nuclear extract	17,900	10,500	0.59	100	1
DNA-cellulose	200	7,350	37	70	63
CUP-1 affinity matrix	0.4	4,200	10,500	40	17,800

^*200 10-cm monolayers of confluent 3T3-L1 preadipocytes.