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Kinetics of the reconstitution of apo-SDH with free zinc (□) and MT-1 (▪). Free zinc (1.7 μM) or MT (0.24 μM) (molar ratio between zinc and apo-SDH of 1.0) was incubated with apo-SDH (1.7 μM) in 0.2 M Tris⋅HCl, pH 7.4. Aliquots (10 μl) were periodically withdrawn from this mixture and assayed for enzymatic activity. Reactivation is expressed as the activity of native SDH recovered.
