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Reconstitution of apo-SDH with free zinc (A) and MT-1 (B). Free zinc or MT-1 was incubated with apo-SDH (1.7 μM) under different ratios of zinc to apo-SDH monomers in 0.2 M Tris⋅HCl, pH 7.4, for 30 min (or 60 min for MT-1). Aliquots (10 μl) were withdrawn from this mixture and assayed for enzymatic activity.
