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. 1998 Mar 31;95(7):3483–3488. doi: 10.1073/pnas.95.7.3483

Figure 4.

Figure 4

Kinetics of the reconstitution of apo-SDH with MT-1 in the presence of GSSG and/or GSH. MT (0.24 μM) (molar ratio between zinc and apo-SDH of 1.0) was incubated with apo-SDH (1.7 μM) in 0.2 M Tris⋅HCl, pH 7.4, in the presence of GSSG and/or GSH. ▪, In the absence of GSH and GSSG; ⧫, in the presence of 3 mM GSSG; ★, in the presence of 3 mM GSSG and 1.5 mM GSH; or □, in the presence of 10 μM GSH. Aliquots (10 μl) were periodically withdrawn from the mixtures and assayed for enzymatic activity.