Figure 5.

Dependence of the reactivation of apo-SDH on the concentration of GSH at a fixed concentration of 3 mM GSSG (A) and on the concentration of GSSG at a fixed concentration of 1.5 mM GSH (B). MT (0.24 μM) was incubated with apo-SDH (1.7 μM) in 0.2 M Tris⋅HCl, pH 7.4, for 60 min at the indicated concentrations of GSH and GSSG. Aliquots (10 μl) were then withdrawn from the mixtures and assayed for enzymatic activity.