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Radiochromatograms of 65Zn-MT-2 with apo-SDH in the absence or presence of GSH and GSSG. MT-2 was incubated with apo-SDH (molar ratio between zinc and apo-SDH of 1.0) for 60 min and then the mixture was analyzed by radiochromatography. (A) In the absence of GSH and GSSG. (B) [GSH] = 1.5 mM, [GSSG] = 3 mM. For the determination of the number of zinc atoms transferred, the radiochromatograms were integrated from fractions 14–17. ▪, MT control; □, MT after the reaction with apo-SDH.
