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Effect of Zn(II) on the reaction of T with DTNB. T, 0.5 μM, was incubated with various concentrations of zinc sulfate in 20 mM Hepes, pH 7.5, for 30 s, and DTNB was added to a final concentration of 50 μM. Relative reactivity of thiols was determined from measurements taken after 30 s.
