Figure 5.

Replication of viruses with N gene translocations. The rearranged viruses were assayed in comparison to N1 (WT) for replication potential by single step growth in BHK-21 cells infected at a multiplicity of 3 pfu. Titers of virus harvested at the indicated intervals were assayed in duplicate, and the results were averaged. Data shown are for replication at 37°C. Replication also was analyzed at 31°C, and viral yields per cell calculated at 36 hr postinfection for single step growth carried out at 37°C or 31°C are shown in the Inset.