Fig. 1.

Enforced expression of Wnt ligands in bone marrow. (A) Diagram of the control MSCV MGirL22Y IRES-GFP vector (Top), the Wnt3a-IRES-GFP vector (Middle), and the Wnt5a-IRES-DsRed vector (Bottom). (B) Average repopulation of whole bone marrow by CD45.1 bone marrow transduced with either the control vector (n = 9), Wnt3a-IRES-GFP (n = 7), or Wnt5a-IRES-DsRed (n = 8). FACS analysis of whole recipient whole bone marrow was performed 16 weeks after transplant. Data represent the pooled results of two independent experiments. Error bars in B–D represent standard deviation, and P values were generated by Student's t test. (C) Average percentage of CD45.1⁺ bone marrow cells positive for GFP (control and Wnt3a vectors) or DsRed (Wnt5a vector). (D) Average percentage of CD45.1⁺ lin⁻ bone marrow cells positive for GFP (control and Wnt3a vectors) or DsRed (Wnt5a vector). (E) PCR was used to amplify a fragment from the GFP cDNA in recipients of bone marrow transduced by Wnt3a-IRES-GFP. PCR plasmid was used as a positive control (+), and DNA isolated from wild-type bone marrow was used as a negative control (−).