Fig. 2.

RHO-1 activity is required to organize myosin in the cortex. (A) Time-lapse images of cortical confocal views of NMY-2-GFP of rga-3/4(RNAi), control, ect-2(RNAi), and cyk-4(RNAi) embryos during polarity establishment. Times (minutes:seconds) are relative to pronuclear appearance. Note that in ect-2(RNAi) embryos, the acto-myosin contractile meshwork does not form, but the myosin foci respond to the polarization signal from the centrosomes and move away from the polarization site. In cyk-4(RNAi) embryos NMY-2 organization appears normal. (B) Magnified section of the cortex showing NMY-2-GFP. (Scale bars: 4.5 μm.)