Fig. 3.

RGA-3/4 control the position of the boundary between anterior and posterior PAR domains. Time-lapse images of control, rga-3/4(RNAi), and cyk-4(RNAi) embryos (after 28–31 h of RNAi incubation) expressing GFP-PAR-2 (green) and mCherry-PAR-6 (red) either during polarity establishment (A) or during the period from anaphase through cytokinesis (B) (SI Movies 4, 5, and 10). One boundary of each GFP-PAR-2 domain (white dot) was tracked manually over time (minutes:seconds). The distance between this boundary and the posterior pole was measured and is expressed as a fraction of total embryo length in percent. Zero percent corresponds to the posterior pole. The distance is shown for control (dark gray bars), rga-3/4(RNAi) (white bars), and cyk-4(RNAi) (light gray bars) embryos. During PAR domain establishment (A) the boundary between the PAR domains becomes positioned more anterior in rga-3/4(RNAi) embryos than in control and cyk-4(RNAi) embryos. Subsequently, this position is unstable in rga-3/4(RNAi) embryos (B). In cyk-4(RNAi) embryos a meiotic PAR-2 domain is formed (A), which often disappears before cytokinesis (B).