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. 2007 Sep 11;104(38):14976–14981. doi: 10.1073/pnas.0706941104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 6. — Codepletion of ECT-2 and RGA-3/4 by RNAi. Shown are time-lapse images of control [klp-1(RNAi)], rga-3/4(RNAi);klp-1(RNAi), ect-2(RNAi), and rga-3/4(RNAi);ect-2(RNAi) embryos expressing GFP-PAR-2 (green) and mCherry-PAR-6 (red). In rga-3/4(RNAi);ect-2(RNAi) embryos (SI Movie 9) contractility is reduced compared with rga-3(RNAi) and increased compared with ect-2(RNAi) embryos. Polar body extrusion failed in rga-3/4(RNAi);ect-2(RNAi) embryos like in ect-2(RNAi) embryos under weaker RNAi conditions (data not shown). (A) Onset of posterior smoothing. (B) Beginning of PAR-2 domain formation. (C) Time point of maximal PCF ingression. (D) Time point of maximal PAR-2 extension. Times are in minutes:seconds.

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