Figure 2.

Time courses for the formation of fluorescent complexes in the absence of Mg²⁺ for the wild-type and mutant T4 DNA polymerases with the G+C-rich 2AP-DNA substrate. A single-exponential curve fit is shown superimposed on the data for the wild-type and D131N-DNA polymerases. The time course for the G255S-DNA polymerase was better described by a double-exponential equation. Fluorescence intensity remained at the background level (equivalent to the fluorescence of a mixture of enzyme, DNA, EDTA, and DTT) in the time course with the D131G-DNA polymerase.