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. 2007 Sep 11;104(38):15028–15033. doi: 10.1073/pnas.0703773104

Fig. 4.

Fig. 4.

Validation of dual-splice-site recognition in human and mouse. RT-PCR analysis of RNA from human brain (b), testis (te), tonsil (to), and thymus (th) tissues (Clontech) and HeLa (H), Weri-Rb1(W), NIH 3T3 (3), and NSC-34 (N) cells. Diagram (A–C) of the general splicing pattern and location of primers (arrowheads) used to detect isoforms that result from the use of the 5′ splice site or 3′ splice site of Smac/Diablo (3′ss, 234 nt; 5′ss, 205 nt) (B) and UBE2C (3′ss, 287 nt; 5′ss, 233 nt) (C). In the case of UBE2C, the exon containing the dual splice site is also skipped to give an additional isoform (skip, 82 nt). (D) Quantitative analysis of RT-PCR results. The histogram represents the percentage of the products that are generated by use of the 5′ splice site.