Fig. 5.

IL-2 production early after infection is regulated by CD4 T cell precursor frequency. The indicated number of CD45.2 TEa cells was transferred to CD45.1 mice that were infected 24 h later with VSV-SED. The mice were injected i.v. with 250 μg of BFA 18 h later. TEa cells in the spleen were analyzed 6 h later by intracellular cytokine staining directly ex vivo. Numbers represent the percentage of TEa CD4 T cells producing IL-2 (Left) or IFN-γ (Right).