Fig. 7.
Y-Ae treatment at the time of infection inhibits generation of memory TEa cells. (A) Recipient mice that received 1 × 105 TEa cells were treated i.p. with 1 μg of Y-Ae or 1 μg of mouse IgG2b before VSV-SED infection. Cells from the spleen and lung were analyzed for the presence of TEa cells at day 5 (primary) or day 23–24 (memory) after infection. Numbers indicate the percentage of the donor TEa population of the total CD4 population (values represent the mean of three mice for primary and eight mice for memory experiments). (B) Graphical representation of data presented in A. *, P < 0.05. (C) Cytokine production by TEa cells in Y-Ae-treated mice. TEa T cells (1 × 105) were transferred into congenic recipients. Before infection with VSV-SED, mice were treated with 1–2 μg of Y-Ae mAb (n = 6) or IgG2b control Ab (n = 3). TEa cells were analyzed 5 days later for intracellular cytokine production after in vitro peptide stimulation.