Table 1.
Summary of tested genes, gene groups, and rat phenotype directed by RNAi
| Genes tested (alternate gene names) | Rat phenotype* (no. of rat mutant lines) |
|---|---|
| Bromodomain proteins | |
| BRD8, BRD12 | None |
| Chromodomain proteins | |
| CRD1 (TFL2, LHP1) | None |
| Chromatin remodeling complex | |
| CHB3, CHB4, CHC1, CHE1 (BSH1), CHR1 (CHA1, DDM1), CHR2 (CHA2), CHR4 (CHA4), CHR5 (CHA5), CHR6 (CHA6), CHR9 (CHA9), CHR10 (CHA10), CHR11 (CHA11), CHR14 (CHA14), CHR15 (CHA15), CHR16 (CHA16), CHR18 (CHA18), CHR19 (CHA19), CHR20 (CHA20), CHR21 (CHA21), CHR22 (CHA22) | CHC1(2), CHR1(1) |
| DNA methyltransferases | |
| DMT1 (MET1, DDM2), DMT2 (MET2), DMT4 (CMT1), DMT5 (CMT2), DMT6(CMT3), DMT7 (DRM2) | DMT1(2), DMT2(1), DMT4(1) |
| Global transcription factors | |
| GTA2, GTC1, GTE1, GTE2, GTE3, GTE4, GTE5, GTE6, GTE7 | GTE2(1), GTE4(2), GTE7(4) |
| Histone acetyltransferases | |
| HAC1, HAC2, HAC4, HAC5, HAF1 (HAC13, GTD1), HAG1 (HAC3), HAG2 (HAC7), HAG3 (HAC8), HAG4 (HAC6), HAG5 (HAC11), HXA1 (HAC9), HXA2 (HAC10) | HAC1(1), HAF1(5), HAG3(2) |
| Histone deacetylases | |
| HDA2, HDA5, HDA6, HDA8, HDA9, HDA14, HDA15, HDA19 (HDA1, HD1), HDT1 (HDA3, HD2A), HDT2, HDT3 (HDA11, HD2c), HDT4 (HDA13, HD2d), SRT1 (HDA12) | HDA15(3), HDA19(2), HDT1(2), HDT2(5) |
| Histone H1 | |
| HON1 (H1–1), HON5 | HON5(1) |
| Methyl-binding domain proteins | |
| MBD1, MBD2, MBD7, MBD8, MBD10, MBD11 | None |
| MAR-binding filament-like protein | |
| MFP1 | None |
| Nucleosome/chromatin assembly factor | |
| NFA1, NFA2, NFA3, NFA4, NFA6, NFB1 (FAS2, MUB3), NFC1, NFC2 (MSI2), NFC4 (MSI4), NFD1(HMGA, HMGa), NFD2, NFD3 (HMGB1), NFD4, NFD5 (HMGD, HMGd), NFD6, NFD7, NFD10 | NFA2(2), NFA3(1) |
| SET domain proteins | |
| SDG1 (CLF, SET1), SDG2 (SET2, ATXR3), SDG3 (SET3, SUVH2), SDG7 (SET7, ASHH3), SDG8 (SET8, ASHH2), SDG9 (SET9, SUVH5), SDG10 (EZA1, SET10), SDG11 (SET11, SUVH10), SDG15 (SET15, ATXR5), SDG17 (SET17, SUVH7), SDG19 (SET19, SUVH3), SDG22 (SET22, SUVH9), SDG25 (SET25, ATXR7), SDG26 (SET26, ASHH1), SDG29 (SET29, ATX5), SDG34 (ATXR6, SET34) | SDG1(1), SDG15(1), SDG22(1), SDG29(1) |
| Silencing group A | |
| SGA1 | SGA1(3) |
| Silencing group B | |
| SGB1, SGB2 | SGB2(1) |
| Silencing group F | |
| SGF1 | None |
| Total: 15 gene groups, 109 genes, 340 lines | 24 genes (46) |
*RNAi directed against genes listed in bold generated a strong rat phenotype; RNAi directed against genes listed without bold generated a weak or variable rat phenotype.