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. Author manuscript; available in PMC: 2007 Sep 19.
Published in final edited form as: Curr Neurovasc Res. 2006 Feb;3(1):25–39. doi: 10.2174/156720206775541741

Fig. (6). Nuclear DNA fragmentation occurs in conjunction with phosphorylation of the retinoblastoma protein (Rb) following NO exposure.

Fig. (6)

(A) Representative fields illustrate the double staining of neurons with p-Rb and TUNEL performed 24 hr following NO exposure (NOC-9, 300 μM). NO exposure resulted in significant p-Rb staining (red). p-Rb labeling (green) also was evident in neuronal cultures exposed to NO. Merged images reveal staining for both proteins in the same neuronal cells. (B) Quantitative results fro either p-Rb alone, TUNEL alone, or combined TUNEL with p-Rb were determined 6 and 24 hr following NO exposure (SNP or NOC-9, 300 μM). p-Rb positive neurons progressively became positive for TUNEL staining over a 24 hr period during NO exposure (*p<0.01 vs. control untreated neurons). To simplify the figures, results for the two NO donors were combined and data were represented as mean ± SEM.