Fig. (8). Inhibition of Rb phosphorylation down-regulates the expression of calreticulin during NO exposure.
A representative Western blot analysis for calreticulin expression following NO exposure is illustrated using 50 μg/lane for neuronal lysates. Rb phosphorylation inhibitors olomoucine (Olo, 50 μM) or butyrolactone (But, 50 μM) were applied to neuronal cultures 24 hr prior to a NO generator (SNP, 300 μM or NOC-9, 300 μM) and total protein extracts were prepared at 24 hr following NO exposure. Olo and But significantly reduced the expression of calreticulin following NO exposure.