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. Author manuscript; available in PMC: 2007 Sep 19.
Published in final edited form as: Mol Cell. 2007 Aug 3;27(3):498–508. doi: 10.1016/j.molcel.2007.07.008

Figure 3. JNK activation by saturated FFA is MLK3 dependent.

Figure 3

(A) Wild-type MEF were treated (16 h) with 0.5 mM palmitic acid. The expression of MLK3 and phosphorylation of the MLK3 T-loop (Thr-277 and Ser281) was examined by immunoblot analysis.

(B - D) Wild-type (WT) or Mlk3−/− MEF were treated (16 h) with 0.5 mM oleic acid (18:1) or 0.5 mM palmitic acid (16:0). The phosphorylation and expression of JNK (B), p38 MAPK (C), and ERK1/2 (D) was examined by immunoblot analysis.