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. Author manuscript; available in PMC: 2008 Jul 4.
Published in final edited form as: Life Sci. 2007 Jun 13;81(4):306–316. doi: 10.1016/j.lfs.2007.05.021

Figure 7.

Figure 7

Immunohistochemistry performed in paraffinized LV tissue sections and visualized by using confocal microscopy. A. Representative photomicrographs (magnification 63x) of LV tissue sections from sham, 1-, 3- and 7-day post-sepsis stained for DNA fragmentation using APO-BrdU TUNEL kit (Invitrogen). TUNEL positive cells are stained green (Alexa Flour 488) in blue nuclei (TO-PRO) along with NOMARSKY DIC (Differential Interference Contrast) images of LV tissue sections.

B. Representative pictomicrographs (magnification 40x) of LV tissues from sham, 1-, 3- and 7-days post-sepsis stained for caspase-3 expression using FITC-conjugated caspase-3 antibody. The cytosol of the LV tissue is stained green (FITC) and the nuclei are counterstained blue with TO-PRO.

C. Representative pictomicrographs (magnifications 200x and 40x) of LV tissues from sham, 1-, 3- and 7-days post-sepsis stained for PARP expression using FITCconjugated caspase-3 antibody. The nucleus of the LV tissue exhibits green (FITC) fluorescence depicting DNA breaks in the blue nuclei which are counterstained with TO-PRO.