Skip to main content
. Author manuscript; available in PMC: 2007 Sep 19.
Published in final edited form as: Curr Neurovasc Res. 2006 Aug;3(3):187–201. doi: 10.2174/156720206778018758

Fig. (1). OGD leads to microglial cell injury that is prevented by erythropoietin (EPO).

Fig. (1)

(A) Microglia were exposed to OGD for 2, 4, 6, 8, and 12 hours and cell survival was determined 24 hours after OGD. The cell survival was progressively decreased over a period of 2, 4, 6, 8, and 12 hours of OGD (* P < 0.01 vs. untreated control). CON = untreated control cultures. (B) Increasing concentrations of EPO from 0.001 to 1000 ng/mL were applied to microglia for a 24-hour period and cell survival was determined by using the trypan blue exclusion method. The cell survival of microglia in the presence of EPO at the concentration 0.001 to 1000 ng/mL was not different from that in the cultures in absence of EPO. (C) EPO, at concentrations of 0.001 to 1000 ng/mL, was applied to the cultures of microglia 1 hour prior to a 6 hour period of OGD and cell survival was assessed 24 hours later. Cytoprotection by EPO during OGD was evident in cultures with 0.1 to 100 ng/mL EPO when compared with cultures exposed to OGD alone (* P < 0.01 vs. OGD). CON = untreated control cultures. (D) EPO (10 ng/mL) was applied to microglia at 2, 4, 6, and 12 hours following a 6 hour period of OGD exposure. Cell survival was evaluated 24 hours following OGD. Application of EPO at 2 and 4 hours after OGD significantly increased microglial survival (* P < 0.01 vs. OGD). In all cases, each data point represents the mean and SEM. Control indicates untreated cultures.