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. Author manuscript; available in PMC: 2007 Sep 19.
Published in final edited form as: Curr Neurovasc Res. 2006 Aug;3(3):187–201. doi: 10.2174/156720206778018758

Fig. (2). Erythropoietin (EPO) prevents apoptotic genomic DNA fragmentation and membrane phosphatidylserine (PS) exposure during OGD in microglia.

Fig. (2)

EPO (10 ng/mL) was applied to microglia 1 hour prior to a 6 hour period of OGD exposure. Apoptotic genomic DNA fragmentation and membrane PS exposure were assessed using TUNEL assay and annexin V phycoerythrin labeling respectively 24 hours after OGD. (A) Representative images for TUNEL illustrate DNA fragmentation in microglia following OGD, but prevention of DNA fragmentation during EPO (10 ng/mL) application. (B) Quantification of data demonstrates that EPO (10 ng/mL) applied to microglia 1 hour prior to a 6 hour period of OGD significantly decreased DNA fragmentation 24 hours after OGD (* P < 0.01 vs. OGD alone). (C) Representative images illustrate cellular membrane PS exposure in microglia by fluorescence (F) light field with 490-nm excitation and 585-nm emission wavelengths 24 hours following OGD exposure. EPO significantly reduced PS staining during OGD exposure. (C) Quantification of data demonstrates that EPO (10 ng/mL) applied to microglia 1 hour prior to a 6 hour period of OGD significantly decreased membrane PS exposure 24 hours later (* P < 0.01 vs. OGD). In all cases, control indicates untreated cultures.