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. Author manuscript; available in PMC: 2007 Sep 19.
Published in final edited form as: Curr Neurovasc Res. 2006 Aug;3(3):187–201. doi: 10.2174/156720206778018758

Fig. (6). Erythropoietin (EPO) phosphorylates glycogen synthase kinase-3β (GSK-3β) through Akt1.

Fig. (6)

Equal amounts of microglial protein extracts (50 μg/lane) were immunoblotted with phospho-GSK-3β antibody 12 hours after a 6 hour period of OGD. EPO (10 ng/mL) applied 1 hour prior to a 6 hour period of OGD significantly increased the expression of p-GSK-3β (inactive form). Transfection of Akt1 siRNA in microglia for 72 hours prior to the OGD insult decreased the efficacy of EPO resulting in a significantly diminished expression of p-GSK-3β (* P < 0.01 vs. Control; †P <0.01 vs. EPO/OGD). Band density was performed using the public domain NIH Image program (http://rsb.info.nih.gov/nih-image). Each data point represents the mean and SEM. CON = untreated control cultures.