Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2008 Jul 20.

Published in final edited form as: J Mol Biol. 2007 May 22;370(4):742–751. doi: 10.1016/j.jmb.2007.05.021

Table 1b.

Kinetic parameters of wild-type and chimeric enzymes with purine nucleosides.

	dA			dG
	k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (M⁻¹s⁻¹)	k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (M⁻¹s⁻¹)

hTK2^a	nd	nd	nd	nd	nd	nd
DmdNK^a	26.2 ± 0.5	315 ± 23	8.3 × 10⁴	12.5 ± 0.4	710 ± 70	1.8 × 10⁴
DH-03	0.45 ± 0.01	1300 ± 190	1.7 × 10²	0.23 ± 0.01	610 ± 70	7.4 × 10²
HD-15	4.6 ± 0.1	200 ± 14	2.2 × 10⁴	5.6 ± 0.1	310 ± 20	1.8 × 10⁴
HD-16	1.2 ± 0.1	1240 ± 170	9.3 × 10²	0.23 ± 0.1	1530 ± 110	1.5 × 10²
DH-02	nd	nd	nd	nd	nd	nd
HDHD-06	nd	nd	nd	nd	nd	nd
HDHD-12	2.5 ± 0.1	1100 ± 60	2.2 × 10³	1.1 ± 0.1	2340 ± 200	4.5 × 10²

^a

Data from Gerth et al.⁶