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. Author manuscript; available in PMC: 2007 Sep 19.
Published in final edited form as: Int Immunol. 2004 Sep 6;16(10):1523–1533. doi: 10.1093/intimm/dxh154

Fig. 5.

Fig. 5

Direct cell–cell contact is required for MHC class II transfer. BLS-CD8 T cells and M-35 EBV cells (1:1) were co-cultured mixed (A) or separated by a semi-permeable membrane (0.4 μm) in a transwell dish (B) for 3 h. In a separate experiment, BLS-CD8 Tcells were co-cultured for 24 h with either M35-EBV cells (C), supernatant from a 2 day old M35-EBV cell culture (D) or exosomes purified from M35-EBV cells. The HLA-DR expression on CD8+ gated BLS-CD8 T cells was measured by flow cytometry and analyzed as described in the legend to Fig. 1, and the DRTI is indicated. Thin lines represent HLA-DR staining of the control BLS-CD8 T cells (not incubated with APC, supernatant or exosomes). These results are representative of three independent experiments.