Fig. 3.

Cellular decay assay of bone marrow cellularity after 10-Gy irradiation. Bone marrow was harvested from femurs and tibias 1 to 24 h after infusion of allogeneic marrow cells. The recipient mice were irradiated with 10-Gy 2 h prior to transplant, thus times indicated reflect the cellularity decay 3, 5, 8, and 26 h after irradiation. Based on the cellularity, an enrichment ratio was calculated; this ratio allows calculating the relative increase of non-irradiated allogeneic homed cells compared to the host bone marrow cells. Using this data, an enrichment ratio was used to correct progenitor assays and secondary engraftment data to allow quantization and comparison between groups (infravide). There were six groups of two mice pooled together; standard error of the mean is shown.