Figure 2. FGF2 enhances Hes5 promoter activity in OP cells.
OP cells were transfected using plasmids with luciferase reporter transcription driven by the promoter region for Hes1 or Hes5. On the following day, the cultures were transferred to defined medium with (+) or without (−) FGF2 for 10 hrs and transcription activity was then measured as relative luciferase units (RLU). FGF2 treatment increased the Hes5 promoter activity approximately 4-fold (*p < 0.001) but did not alter Hes1 promoter (p > 0.05). Values (mean ± standard error of the mean) are from duplicate wells of three independent experiments.