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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Neuron. 2007 Feb 1;53(3):353–369. doi: 10.1016/j.neuron.2006.12.010

Figure 3.

Figure 3

Oligo-1 mediates repression of maxillar palp Or genes in the antenna. (A) Mutation of Oligo-1 in the 450 bp Or85e promoter leads to misexpression in antennal cells (left panels) and a reduction in maxillary palp expression (right panels) in flies containing 0.45(Oligo)-GAL4 and UAS-lacZ. At least 5 independent transgenic lines were tested for both wild-type and mutant constructs. (B) The Or71a 5'-GAL4 construct drives expression of lacZ in the antenna and maxillary palp (left). In Or71a 5'+3'-GAL4 flies, the addition of the 3' region, which contains two Oligo-1 elements, represses expression in the antenna (center). Mutation of the Oligo-1 elements in the 3' region leads to antennal expression (right). At least 2 independent transgenic lines were tested for each construct. Each construct contains 2.3 kb of DNA from the 5' region of Or71a. The distance between the start and stop codons of Or71a is 1.24 kb, compared to 1.6 kb for Or85e. (C-E) Maxillary palps co-stained for GFP (green) and Or71a RNA and from flies containing (C) Or71a 5'-GAL4; (D) Or71a 5'+3'-GAL4; and (E) Or71a 5'+3'(oligo)-GAL4.

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