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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Neuron. 2007 Feb 1;53(3):353–369. doi: 10.1016/j.neuron.2006.12.010

Figure 4.

Figure 4

pb2A-2 is a neuron-specific positive regulatory element. (A) The pb2A-1 and pb2A-2 elements, indicated as “1” and “2”, respectively. (B) Mutational analysis of the Or85e promoter. Micrographs show maxillary palps of flies containing UAS-GFP and Or85e 0.45kb-GAL4 (top), Or85e(pb2A-1)-GAL4 (middle), and Or85e(pb2A-2)-GAL4 (bottom). At least 9 independent transgenic lines were tested for each of the mutant constructs. (C) Seven copies of the pb2A-2 element (green boxes) fused to the Or85e 90 bp basal promoter (above) or a TATA box (below) drives expression in the maxillary palp. Dyad-1 sequences in the forward strand are indicated in red. Confocal micrographs show maxillary palps with each GAL4 driver. Insets show images of neurons taken at higher magnification; arrowheads indicate dendrites extending from neuronal cell bodies. Multiple copies of pb2A-2 were used since a single copy was not sufficient to drive expression in a shortened promoter (Figure 2C; 0.35 kb construct).

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