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. 1998 Mar 31;95(7):3519–3524. doi: 10.1073/pnas.95.7.3519

Figure 5.

Figure 5

HDAC activity is important for targeted transcriptional repression by HDAC1 in vivo. (A) GAL4-driven luciferase reporter construct and HDAC1 wild-type (WT) and mutant (H141A) GAL4-VP16 DNA-binding expression constructs. (B) Extracts from transfected 293 cells were prepared and luciferase and β-galactosidase activities were assayed according to manufacturer’s directions (Promega). Luciferase values (relative light units, r.l.u.) were normalized for transfection efficiency by dividing by β-galactosidase activity. Cells were treated with or without 10 nM TPX as described. Between individual experiments, TPX-induced derepression of WT-GAL4-VP16 averaged 7.5-fold as compared with an average of 2.5 for H141A-GAL4-VP16. r.l.u. by GAL4-VP16 are 300–600× higher than WT-GAL4-VP16 and derepression by TPX is generally 1.5–2.0-fold (data not shown).