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Fits to the Hill equation for β9′UAG+THG73 (74 mer)/-dCA (76 mer) and β9′Q. Filled circles are β9′UAG+THG73 (74 mer) (16 ng per oocyte), open squares are β9′Q, and filled triangles are β9′UAG+THG73-dCA (76 mer) (5 ng per oocyte). EC50 values are 0.24 ± 0.006, 0.31 ± 0.02, and 0.88 ± 0.08 μM ACh, respectively. In each experiment n >3 oocytes.
