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. 2007 Oct;13(10):1715–1722. doi: 10.1261/rna.667607

FIGURE 4.

FIGURE 4.

Amber, opal, and frameshift suppressor tRNAs suppression at α149. All tRNA-W (7.5 ng per oocyte) average current was normalized to THG73-W average current. TQAS′-W, TQOpS′-W, and TQOpS-W were performed twice and error bars represent the standard error. Total oocytes tested are 161 oocytes, where 16>n>5 for each experiment. Bar colors are the same as in Fig. 2. Amber suppression (THG73-W, TQAS′-W, or TQAS-W) is the most efficient. Opal suppression is variable, with TQOpS′-W and TQOpS-W suppressing 48% and 21%, respectively, of THG73-W in Nasco oocytes. YFFSCCCG-W suppresses less than YFaFSACCC-W, as previously seen in Xenopus oocytes (Rodriguez et al. 2006). TQAS′-W and TQAS-W show increased suppression compared to THG73-W.