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. Author manuscript; available in PMC: 2008 Aug 24.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Jun 26;360(2):507–512. doi: 10.1016/j.bbrc.2007.06.090

Fig. 2.

Fig. 2

PP1α dephosphorylates BRCA1 at multiple serine residues. (A) Recombinant PP1α but not PP2A dephosphorylates S1524 and S1423 of BRCA1. Hyperphosphorylated BRCA1 was immunoprecipitated from 293T cells irradiated with 5 Gy of γ-radiation (IR) or treated with 1 mM of HU for 1h, and used for the phosphatase assay using buffer only (−), recombinant PP1α, or purified PP2A. (B) Overexpression of PP1αGFPdecreases BRCA1 S1423 phosphorylation both at the basal level (C) and after DNA damage induced by HU.