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The number of RA4-positive cells at different time points after the addition of bFGF to E5 RPE cell cultures. A dramatic increase in the number of RA4-positive cells was detected only after 48 h of bFGF treatment. No statistically significant increase in the number of RA4-positive cells was detected when the culture was treated with bFGF for 24 h before fixation as compared to control (CK).
