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(A) The chemical structure of the Q-nucleoside present in the anticodon loop of tRNATyr. (B) An outline of an experimental scheme leading to formation of a complex between N-acetyl-aminoacyl-tRNA and the large ribosomal subunit and capturing the complex on the resin. N-Acetyl-tyrosyl residue is shown as a solid circle, biotin as a triangle, the large ribosomal subunit as a gray oval, and the avidin resin as a hatched semicircle.
