Figure 1.

Coomassie brilliant blue stained SDS-polyacrylamide gel showing pattern of polypeptide fragments produced by limited trypsin and chymotrypsin digestion of NaeI protein. The time of digestion is indicated along the tops of the lanes. The M_r of protease-resistant fragments analyzed in this study are indicated alongside the gel image and are based on the molecular weight (MW) markers glutamic dehydrogenase (55,561 Da), maltose-binding protein (42,710 Da), lactate dehydrogenase (36,487 Da), triosephosphate isomerase (26,625 Da), trypsin inhibitor (20,040–20,167 Da), lysozyme (14,313 Da), and aprotinin (6,517 Da). The digestion pattern at 0 min is caused by the amount of digestion taking place during mixing and sampling.