Figure 5.

Analytical ultracentrifuge determination of molecular weight of maltose-binding protein fusions with NaeI, and its N-terminal and C-terminal domains. Centrifugation was at 50,000 rpm; A₂₈₀ boundary profiles are shown at 5, 10, 15, 20, 25, 30, 35, 40, and 45 min (from left to right). x-axis shows the radius of the sample cell (from left to right is the direction of centrifugal force); y-axis shows the A₂₈₀ of the solution, measured through the sample cell. (A) MBP∷NaeI; (B) MBP∷NaeI(169–317); (C) MPB∷NaeI(1–145).